Fluorescence Filter

Fluorescence filters are specialized optical filters designed for fluorescence microscopes and other fluorescence detection systems. Their primary function is to separate excitation light from emission light, ensuring that only the target fluorescence signal is detected, thereby improving the signal-to-noise ratio and clarity of fluorescence imaging. Fluorescence filters typically consist of an excitation filter, an emission filter, and a dichroic mirror, which work together to achieve efficient separation of fluorescence signals.

Technical Parameters:

• Center Wavelength (CWL): Customized based on the excitation and emission wavelengths of fluorescent dyes.
• Bandwidth (FWHM): Typically 10nm-50nm, adjustable according to requirements.
• Peak Transmittance (Tmax): ≥90%.
• Blocking Rate (OD): ≥OD5 (out-of-band wavelengths).
• Substrate Materials: K9, BK7, quartz, etc.
• Surface Quality: 20/10.
• Size: Customizable according to requirements.

Components of Fluorescence Filters:

Excitation Filter:

Selectively allows excitation light to pass through, stimulating the fluorescent sample.

Typically positioned between the light source and the sample.

Emission Filter:

Selectively allows the fluorescence emitted by the sample to pass through while blocking excitation light and other stray light.

Typically positioned between the sample and the detector.

Dichroic Mirror:

Reflects excitation light and transmits emission light, achieving separation of excitation and emission light.

Usually placed at a 45° angle in the optical path.

Applications:

Fluorescence Microscopy:

Used for fluorescence imaging in cell biology, pathology, immunology, and other fields.

Supports multi-color fluorescence labeling (e.g., DAPI, FITC, TRITC, Cy5).

Flow Cytometry:  Used for cell sorting and fluorescence signal detection.

Gene Sequencing: Used in DNA sequencers for the separation and detection of fluorescence signals.

Environmental Monitoring: Used in water quality testing and microbial analysis with fluorescence labeling techniques.

Drug Development: Used for high-throughput screening and fluorescence labeling experiments.

Customization Services:

We offer comprehensive customization services to tailor fluorescence filters according to your experimental needs:

Center Wavelength and Bandwidth: Customized based on the excitation and emission spectra of fluorescent dyes. 

Size and Shape: Supports various shapes and sizes, including round, square, and rectangular. 

Multi-Band Filters: Supports multi-color fluorescence detection for complex multi-labeling experiments.